The Carriers of Native Vitamin B12 in Normal Human Serum-Reference-Cited by-同舟云学术

The Carriers of Native Vitamin B12 in Normal Human Serum

Published:1977-11-01 Issue:5 Volume:53 Page:453-457
ISSN:0301-0538
Container-title:Clinical Science
language:en
Short-container-title:

Author:

Hall C. A.¹

Affiliation:

1. Hematology Research Laboratory, Veterans Administration Hospital, and Department of Medicine, Albany Medical College of Union University, Albany, New York, U.S.A.

Abstract

1. After fractionation of the vitamin B12-binding proteins of ten normal sera the components containing transcobalamin II and R-type binders of vitamin B12 respectively were studied for endogenous vitamin B12 content by two distinct systems of vitamin B12 assay. 2. The measurements of total serum vitamin B12 by either bioassay with Euglena gracilis or a radioisotope dilution assay agreed closely. 3. The native vitamin B12 carried by transcobalamin II was higher as measured by bioassay than by isotope dilution assay. 4. The presence of the transcobalamin II fraction of human serum altered the key reaction between the binding reagent of the isotope dilution assay, so that this assay failed to measure vitamin B12 quantitatively. 5. Probably, the mean fraction of plasma vitamin B12 carried by transcobalamin II is in the range 20–30%.

Publisher

Portland Press Ltd.

Subject

General Medicine

Link

http://portlandpress.com/clinsci/article-pdf/53/5/453/739972/cs0530453.pdf

Cited by 36 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Relationship between cobalt transporter II gene rs9606756 site mutant and serum homocysteine level and recurrent cerebral infarction in young and middle-aged people;Minerva Medica;2021-04

2. Oral vitamin B-12 versus intramuscular vitamin B-12, for vitamin B-12 deficiency;COCHRANE DB SYST REV;2018

3. The FUT2 secretor variant p.Trp154Ter influences serum vitamin B12 concentration via holo-haptocorrin, but not holo-transcobalamin, and is associated with haptocorrin glycosylation;Human Molecular Genetics;2017-10-12

4. A missense mutation in TCN2 is associated with decreased risk for congenital heart defects and may increase cellular uptake of vitamin B12 via Megalin;Oncotarget;2017-07-19

5. Vitamin B 12 Deficiency in Vegetarians;Vegetarian and Plant-Based Diets in Health and Disease Prevention;2017