A study by polyacrylamide-gel electrophoresis of the effect of proteolysis on Micrococcus lysodeikticus polynucleotide phosphorylase-Reference-Cited by-同舟云学术

A study by polyacrylamide-gel electrophoresis of the effect of proteolysis on Micrococcus lysodeikticus polynucleotide phosphorylase

Published:1968-12-01 Issue:3 Volume:110 Page:475-479
ISSN:0306-3283
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Fitt P. S.¹,Fitt E. A.¹,Wille H.¹

Affiliation:

1. Department of Biochemistry, Faculty of Medicine, University of Ottawa, Ottawa 2, Ont., Canada

Abstract

1. Trypsin digestion of Micrococcus lysodeikticus polynucleotide phosphorylase (nucleoside diphosphate–polynucleotide nucleotidyltransferase) causes a progressive increase in electrophoretic mobility in polyacrylamide gels of the single active degradation product. 2. A marked increase in primer requirement for CDP polymerization occurs before a more mobile product is formed. 3. α-Chymotrypsin digestion yields a product that separates into several active species on polyacrylamide-gel electrophoretograms. 4. No separation of ADP-and CDP-polymerization activities occurs during electrophoresis after either trypsin or α-chymotrypsin treatment.

Publisher

Portland Press Ltd.

Link

https://portlandpress.com/biochemj/article-pdf/110/3/475/762212/bj1100475.pdf

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