Thymidine kinase of rat liver. Activation by phospholipase C and subcellular distribution in the liver

Author:

Stirpe F.1,La Placa M.2

Affiliation:

1. Istituto di Patologia generale, 40126 Bologna, Italy

2. Seconda Cattedra di Microbiologia della Università di Bologna, 40126 Bologna, Italy

Abstract

1. The thymidine kinase activity of rat liver is greatly enhanced on addition of phospholipase C to the assay mixture. 2. Most of the thymidine kinase activity of the liver is recovered in the mitochondrial and in the impure ‘nuclear’ fractions. No activity was detected in purified nuclei prepared in high-density sucrose. 3. A substantial thymidine kinase activity could be detected, with the aid of phospholipase C, in all rat tissues examined.

Publisher

Portland Press Ltd.

Cited by 12 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. COUNTING RADIOACTIVE NUCLEOTIDES ON ANION EXCHANGE PAPER DISKS: RECENT IMPROVEMENTS;Liquid Scintillation Counting, Recent Applications and Development;1980

2. Deoxythymidine kinase activity of human implanted sponge connective tissue in zinc deficiency.;American Journal of Physiology-Endocrinology and Metabolism;1979-03-01

3. An improved method of counting radioactive nucleotides on DEAE-paper disks;Analytical Biochemistry;1977-05

4. Rapid decline in thymidine kinase activity during differentiation ofNaegleria;Journal of Cellular Physiology;1977-02

5. The presence of a nucleoside phosphotransferase and the absence of a thymidine kinase in Tetrahymena;Comparative Biochemistry and Physiology Part B: Comparative Biochemistry;1977-01

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