Protein–polysaccharide of chicken cartilage and chondrocyte cell cultures

Abstract

The nature of the matrix produced by embryonic chicken chondrocytes in cell culture was studied, and compared with adult and embryonic chicken cartilage. Adult chicken cartilage contains a protein–polysaccharide easily extracted with EDTA–sodium chloride at 4°C. Purification of this macromolecule on Bio-Gel P-300 and Bio-Gel A-50m yielded a progressively more homogeneous species in the ultracentrifuge. It contained mostly chondroitin 4-sulphate, some chondroitin 6-sulphate, and keratan sulphate. Embryonic chicken cartilage was previously shown to contain mostly chondroitin 4-sulphate, some chondroitin 6-sulphate and essentially no keratan sulphate. The matrix produced in chondrocyte cell cultures was shown to contain a protein–polysaccharide with alkali-labile linkages of chondroitin 4-sulphate to the protein core. A fraction was isolated from the matrix with many properties of keratan sulphate.