What can we learn from highly purified neutrophils?

Author:

Sabroe I.1,Prince L.R.1,Dower S.K.1,Walmsley S.R.2,Chilvers E.R2,Whyte M.K.B.1

Affiliation:

1. Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield S10 2JF, U.K.

2. Respiratory Medicine Division, Department of Medicine, University of Cambridge School of Clinical Medicine, Addenbrooke's and Papworth Hospitals, Cambridge, U.K.

Abstract

Neutrophil purification has traditionally been performed by centrifugation of leucocytes through density gradients. These reliable methods produce populations that are typically >95% pure neutrophils, and have allowed the widespread study of the function of these cells. Our recent work has suggested that residual monocytes may play a more important role than has been previously realized, and suggest that for some functional experiments, further purification of cells is required to understand fully the neutrophil phenotype.

Publisher

Portland Press Ltd.

Subject

Biochemistry

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