Author:
Khalfan H,Abuknesha R,Robinson D
Abstract
A method for the preparation of casein labelled with the 4-methylumbelliferyl fluorophore is described, and the product was used as a fluorigenic macromolecular substrate for a sensitive assay of the activity of proteinases. Nanogram quantities of trypsin, chymotrypsin, elastase and cathepsin D can be detected, but the substrate is unaffected by cathepsin B.
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
13 articles.
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