Enzyme purification by hydrophobic chromatography: an alternative approach illustrated in the purification of aspartate transcarbamoylase from wheat germ (Short Communication)-Reference-Cited by-同舟云学术

Enzyme purification by hydrophobic chromatography: an alternative approach illustrated in the purification of aspartate transcarbamoylase from wheat germ (Short Communication)

Published:1974-01-01 Issue:1 Volume:137 Page:127-130
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Yon Robert J.¹

Affiliation:

1. School of Biological Sciences, Thames Polytechnic, Wellington Street, London SE18 6PF, U.K.

Abstract

Two adsorbents containing similar numbers of hydrocarbon (C10) chains but different numbers of carboxyl groups were made by chemical modification of Sepharose. The use of these adsorbents to purify proteins, under conditions where hydrophobic adsorption is partly resisted by electrostatic repulsion, is illustrated in the purification of aspartate transcarbamoylase (EC 2.1.3.2) from wheat germ.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

http://portlandpress.com/biochemj/article-pdf/137/1/127/561439/bj1370127.pdf

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