A novel protein, amyloid precursor-like protein 2, is present in human brain, cerebrospinal fluid and conditioned media-Reference-Cited by-同舟云学术

A novel protein, amyloid precursor-like protein 2, is present in human brain, cerebrospinal fluid and conditioned media

Published:1995-08-15 Issue:1 Volume:310 Page:95-99
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Webster M T¹,Groome N²,Francis P T¹²²,Pearce B R³,Sherriff F E⁴,Thinakaran G⁵,Felsenstein K M⁶,Wasco W⁷,Tanzi R E⁷,Bowen D M¹

Affiliation:

1. Miriam Marks Department of Neurochemistry, Institute of Neurology, London, U.K.

2. School of Biological and Molecular Science, Oxford Brookes University, Gipsy Lane, Headington, Oxford OX3 OBP, U.K.

3. Department of Pharmacology, School of Pharmacy, 29-31 Brunswick Square, London WClN 1AX, U.K.

4. Department of Neuropathology, University of Oxford, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, U.K.

5. Department of Pathology and Neuropathology Laboratory, The Johns Hopkins University School of Medicine, Baltimore, MD 21205-2196, U.S.A.

6. CNS Department of Biophysics and Molecular Biology, Bristol Myers Squibb Pharmaceutical Research Institute, Wallingford, CT 06492-7660, U.S.A.

7. Department of Neurology, Harvard Medical School, Boston, MA 02129, U.S.A.

Abstract

A monoclonal antibody, 3B11, was raised to a novel protein, amyloid precursor-like protein 2, which did not recognize amyloid precursor protein. Multiple bands were detected in human brain fractions and cell lysate by Western blotting, indicating the presence of isoforms, 3B11 immunoreactivity was also detected in cerebrospinal fluid and conditioned medium, indicating that the protein is secreted. Immunocytochemistry revealed 3B11 immunoreactivity in sections of human brain.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/310/1/95/728469/bj3100095.pdf

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