Epidemiology of An Outbreak of Drug-Resistant Tuberculosis in the U.K. Using Restriction Fragment Length Polymorphism

Author:

Goyal M.1,Ormerod L. P.2,Shaw R. J.1

Affiliation:

1. Department of Respiratory Medicine, St Mary's Hospital Medical School, London, U.K.

2. Chest Clinic, Blackburn Royal Infirmary, Blackburn, Lancashire, U.K.

Abstract

1. Drug-resistant tuberculosis is a growing health care problem. When a series of cases occur, it is essential to know if patients with multidrug-resistant disease represent one or a number of separate outbreaks. 2. The epidemiology of an outbreak of isoniazid- and streptomycin-resistant tuberculosis in Blackburn was studied by restriction fragment length polymorphism using a probe for the IS6110 DNA sequence. 3. Mycobacterium tuberculosis from four cases of isoniazid- and streptomycin-resistant disease had an identical restriction fragment length polymorphism pattern. This pattern was not shared by drug-sensitive isolates of M. tuberculosis obtained from Blackburn (n = 8) or London (n = 13) or a M. tuberculosis isolate from a fifth Blackburn case which was resistant to isoniazid alone. 4. This methodology confirmed that all four cases of isoniazid- and streptomycin-resistant disease were part of a single epidemiologically related outbreak of drug-resistant disease. This study demonstrates how the epidemiology of an outbreak of multidrug-resistant tuberculosis in the U.K. can be confirmed by restriction fragment length polymorphism.

Publisher

Portland Press Ltd.

Subject

General Medicine

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