Short-term radiorespirometry of cell suspensions

Abstract

1. An apparatus and method are described with which the oxidation of labelled substrates to14CO2 by cell suspensions may be examined. 2. The use of high-specific-radioactivity substrates at low concentration together with frequent quantitative collection of CO2 permit a more detailed analysis of the appearance of various substrate carbon atoms as CO2 than is possible by existing techniques. 3. Typical experiments with various cell types are reported, in which pathways of glucose oxidation are examined.