Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus

Author:

Wagner Michaela1,Berkner Silvia2,Ajon Malgorzata3,Driessen Arnold J.M.3,Lipps Georg4,Albers Sonja-Verena13

Affiliation:

1. Max-Planck-Institute for Terrestrial Microbiology, Karl-von Frisch-Strasse 1, 35043 Marburg, Germany

2. Department of Biochemistry, University of Bayreuth, 95447 Bayreuth, Germany

3. Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands

4. University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Gründenstrasse 40, 4132 Muttenz, Switzerland

Abstract

Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind. In the present paper, we discuss the latest progress in understanding recombination events of exogenous DNA into the chromosomes of Sulfolobus solfataricus and Sulfolobus acidocaldarius and their application in the construction of targeted-deletion mutant strains.

Publisher

Portland Press Ltd.

Subject

Biochemistry

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