Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate-Reference-Cited by-同舟云学术

Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate

Published:1990-02-01 Issue:3 Volume:265 Page:899-902
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Hawkes T R¹,Lewis T¹,Coggins J R²,Mousdale D M²,Lowe D J³,Thorneley R N³

Affiliation:

1. I.C.I. Agrochemical, Jealotts Hill Research Station, Bracknell, Berks. RG12 6EY, U.K.

2. Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, U.K.

3. A.F.R.C. Institute of Plant Science Research, Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, U.K.

Abstract

The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25 degrees C at pH 7.5. No pre-steady-state ‘burst’ or ‘lag’ phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/265/3/899/627951/bj2650899.pdf

Cited by 37 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Multifunctional Enzymes as Targets for the Treatment of Tuberculosis: Paving the Way for New Anti-TB Drugs;Current Medicinal Chemistry;2021-09-09

2. Antileishmanial Activity of Lignans, Neolignans, and Other Plant Phenols;Progress in the Chemistry of Organic Natural Products 115;2021

3. Comparative Analysis of Mono- and Bifunctional Chorismate Synthases in Escherichia coli Cells Capable and Incapable of Phenylalanine Production;Applied Biochemistry and Microbiology;2017-12

4. A HR-MS Based Method for the Determination of Chorismate Synthase Activity;Protein & Peptide Letters;2017-01-27

5. Secondary Metabolism and the Rationale for Systems Manipulation;Reference Series in Phytochemistry;2017