Development and validation of an immune-related gene prognostic model for stomach adenocarcinoma

Abstract

Abstract Purpose: Stomach adenocarcinoma (STAD) is one of the most common malignant tumors, and its occurrence and prognosis are closely related to inflammation. The aim of the present study was to identify gene signatures and construct an immune-related gene (IRG) prognostic model in STAD using bioinformatics analysis. Methods: RNA sequencing data from healthy samples and samples with STAD, IRGs, and transcription factors were analyzed. The hub IRGs were identified using univariate and multivariate Cox regression analyses. Using the hub IRGs, we constructed an IRG prognostic model. The relationships between IRG prognostic models and clinical data were tested. Results: A total of 289 differentially expressed IRGs and 20 prognostic IRGs were screened with a threshold of P<0.05. Through multivariate stepwise Cox regression analysis, we developed a prognostic model based on seven IRGs. The prognostic model was validated using a GEO dataset (GSE 84437). The IRGs were significantly correlated with the clinical outcomes (age, histological grade, N, and M stage) of STAD patients. The infiltration abundances of dendritic cells and macrophages were higher in the high-risk group than in the low-risk group. Conclusions: Our results provide novel insights into the pathogenesis of STAD. An IRG prognostic model based on seven IRGs exhibited the predictive value, and have potential application value in clinical decision-making and individualized treatment.