Affiliation:
1. Agricultural Research Council Unit of Developmental Botany, 181A Huntingdon Road, Cambridge CB3 0DY, U.K.
Abstract
Broad-bean 5S rRNA labelled with32P was digested separately with T1 and pancreatic A ribonucleases and the resulting oligonucleotides (20 and 18 respectively) were fractionated by two-dimensional electrophoresis. The oligonucleotides were analysed further and 32 of them have been completely sequenced. They were compared with those of 5S rRNA from dwarf bean, sunflower, tomato and rye. Sequence differences were found at both the 3′- and 5′-termini and at up to nine other positions. Most base substitutions were transitions between C and U. In common with other 5S rRNA species that have been analysed the ends of the molecule in each plant species have complementary sequences.
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
26 articles.
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1. Nucleotide Sequences of Wheat-Embryo Cytosol 5-S and 5.8-S Ribosomal Ribonucleic Acids;European Journal of Biochemistry;2005-03-03
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