N6-methyladenosine (m6A)-connected lncRNAs are linked to survival and immune infiltration in glioma patients

Author:

Wu Wei Jun123456,Xiao Feng1234,Xiong Yaping6,Sun Gu Feng1234,Guo Yun1234,Zhou Xiang1234,Hu Guo Wen1,Huang Kai1234,Guo Hua1234ORCID

Affiliation:

1. 1Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China

2. 2Jiangxi Key Laboratory of Neurological Tumors and Cerebrovascular Diseases, Nanchang, China

3. 3Jiangxi Health Commission Key Laboratory of Neurological Medicine, Nanchang, China

4. 4Institute of Neuroscience, Nanchang University, Nanchang, China

5. 5Department of Neurosurgery, Yichun People’s Hospital, Yichun, Jiangxi, China

6. 6Departments of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China

Abstract

Abstract Background: The connection between m6A-assiociateed lncRNAs and prognosis has been demonstrated in multiple types of tumors. However, potential roles of m6A-assiociateed lncRNAs in glioma is still rare. Methods: We implemented consensus cluster analysis to group the downloaded samples into two subtypes. The least absolute shrinkage and selection operator (LASSO) analysis was used to create a risk model. Additionally, the conjunction between m6A-related lncRNAs and immune cells infiltration was explored by conducting the R package. Ultimately, we inspected the underlying downstream pathways of the two subtypes by performing Gene Set Enrichment Analysis (GSEA). The expression level of m6A-connected lncRNAs in glioma were examined by conducting in vitro experiments. Results: We ascertained two subtypes of glioma in line with the consensus clustering of m6A-associated lncRNAs. We confirmed that age, grade, and IDH are related to the two subtypes. Additionally, the immune cells infiltration and immune checkpoint molecules of the two clusters were discussed. A risk signature including AL359643.3, AL445524.1, AL162231.2, AL117332.1, AP001486.2, POLR2J4, AC120036.4, LINC00641, LINC00900, CRNDE, and AL158212.3, was identified using the Cox regression and LASSO analyses. We also verified the prognostic value and discussed the immune cells infiltration and immune checkpoint molecules of the risk signature. In vitro experiments verified that the m6A-associated lncRNAs was abnormally expressed in glioma. Conclusion: We elaborated the significant role of m6A-connected lncRNAs in glioma prognosis and immune infiltration and suggest that these key regulators may serve as underlying therapeutic targets to build up the efficacy of glioma immunotherapy.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

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