Transcriptional regulation of muscle fatty acid-binding protein

Author:

Carey J O1,Neufer P D1,Farrar R P2,Veerkamp J H3,Dohm G L1

Affiliation:

1. Department of Biochemistry, East Carolina University School of Medicine, Greenville, NC 27858, U.S.A.

2. Department of Kinesiology, University of Texas, Austin, TX 78712, U.S.A.

3. Department of Biochemistry, University of Nijmegen, NL-6500 HB Nijmegen, The Netherlands

Abstract

Heart fatty acid-binding protein (H-FABP) is present in a wide variety of tissues but is found in the highest concentration in cardiac and red skeletal muscle. It has been proposed that the expression of H-FABP correlates directly with the fatty acid-oxidative capacity of the tissue. In the present study, the expression of H-FABP was measured in red and white skeletal muscle under two conditions in which fatty acid utilization is known to be increased: streptozotocin-induced diabetes and fasting. Protein concentration, mRNA concentration and transcription rate were measured under both conditions. The level of both protein and mRNA increased approximately 2-fold under each condition. The transcription rate was higher in red skeletal muscle than in white muscle, was increased 2-fold during fasting, but was unchanged by streptozotocin-induced diabetes. In addition to supporting the hypothesis that H-FABP is induced during conditions of increased fatty acid utilization, these findings demonstrate that the regulation of H-FABP expression may or may not be at the level of transcription depending on the stimulus.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

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