The reaction of diethyl pyrocarbonate with pyruvate kinase-Reference-Cited by-同舟云学术

The reaction of diethyl pyrocarbonate with pyruvate kinase

Published:1974-02-01 Issue:2 Volume:137 Page:405-407
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Dann Leighton G.¹,Britton Hubert G.¹

Affiliation:

1. Department of Physiology, St. Mary's Hospital Medical School, London W2 1PG, U.K.

Abstract

Diethyl pyrocarbonate inactivates muscle pyruvate kinase with the substitution of 3–4 histidine residues per subunit. Phosphoenolpyruvate, ATP and ADP to a lesser extent, and Mg2+ and pyruvate to a small extent, protect against inactivation.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/137/2/405/562131/bj1370405.pdf

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