Atomic force microscopy of gastric mucin and chitosan mucoadhesive systems

Author:

DEACON Matthew P.12,McGURK Simon2,ROBERTS Clive J.2,WILLIAMS Phillip M.2,TENDLER Saul J. B.2,DAVIES Martyn C.2,DAVIS S. S. (Bob)2,HARDING Stephen E.1

Affiliation:

1. University of Nottingham, NCMH Physical Biochemistry Laboratory, School of Biosciences, Sutton Bonington LE12 5RD, U.K.

2. University of Nottingham, School of Pharmacy, University Park, Nottingham NG7 2RD, U.K.

Abstract

Atomic force microscopy has been utilized to probe, at a molecular level, the interaction between purified pig gastric mucin (PGM) and a mucoadhesive cationic polymer, chitosan (sea cure 210+), with a low degree (approx. 11%) of acetylation. Images were produced detailing the structures of both PGM and chitosan in 0.1 M acetate buffer (pH 4.5), followed by the complex of the two structures in the same buffer. PGM in 0.1 M acetate buffer revealed long linear filamentous structures, consistent with earlier electron microscopy and scanning tunnelling micoscopy studies. The chitosan molecules also adopted a linear conformation in the same buffer, although with a smaller average length and diameter. They appeared to adopt a stiff-coil conformation consistent with earlier hydrodynamic measurements. The complexes formed after mixing PGM and chitosan together revealed large aggregates. In 0.1 M ionic strength buffer they were of the order of 0.7 μm in diameter, consistent with previous electron microscopy studies. The effect of ionic strength of the buffer on the structure of the complex was also studied and, together with molecular hydrodynamic data, demonstrates that the interaction is principally electrostatic in nature.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

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