Effect of bis-(p-nitrophenyl) phosphate on the biosynthesis and the utilization of lipid-intermediates

Abstract

Incubations of rat spleen lymphocytes with the required labelled nucleotide sugars lead to the formation of the various lipid-intermediates involved in the N-glycosylation of proteins. The effect of bis-(p-nitrophenyl) phosphate on the different reactions involved in the dolichol pathway has been studied. Although dolichyl phosphate mannose, dolichyl phosphate glucose and dolichyl diphosphate N-acetylglucosamine synthesis is not affected at all by bis-(p-nitrophenyl) phosphate (20 mM), this product inhibits completely the addition of the second N-acetylglucosamine residue on the dolichyl diphosphate N-acetylglucosamine acceptor. The addition of the five innermost mannose residues from GDP-mannose as donor is also strongly abolished. However, the addition of the more distal sugars, i.e. the four mannose residues using dolichyl phosphate mannose as donors and the additional glucose residues are only slightly affected. The reactions involved in the utilization of dolichyl diphosphate oligosaccharide, i.e. transfer to the proteins or degradation into soluble phospho-oligosaccharides, are also strongly inhibited. Thus bis-(p-nitrophenyl) phosphate appears to affect only the reactions involving the presence of dolichyl diphosphate sugar as substrate.