Author:
Vodop'janov S.O.,Vodop'janov A.S.,Pisanov R.V.,Ivanov S.A.,Mishan'kin B.N.,Olejnikov I.P.
Abstract
The aim of the study was to analyze the expression of V. cholerae genes that are part of the VcB island
by means of full-genomic sequencing of the transcriptome. The VcB island is localized on the second
chromosome in all toxigenic vibrios studied and is absent in the atoxigenic apiliated strains. Two strains
of V. cholerae O1 ctxA+ tcpA+ and one strain V. cholerae O1 ctxA– tcpA– were studied. The pool of
total RNA vibrios was isolated by a technique based on differential precipitation in the presence of
lithium ions. In the total pool of sequenced RNA, RNA encoded in the order of 3 500 by known cholera
vibrio genes was identified. In a pool of total RNA from two ctx + tcpA + strains RNA transcripts were
found for the five genes included in the VcB island, excluding the VCA0282 gene, previously identified
as the ISVch5-transposase. In the the pool of total RNA from the ctx– tcpA– strain no transcripts of the
two genes previously described as VCA0282-transposase and VCA0283 were detected. A possible
explanation may be the existence of copies of these genes in other parts of the genome of the ctxA– tcpA–
V. cholerae.
Publisher
Federal Center for Hygiene and Epidemiology
Reference13 articles.
1. Vodop'yanov A.S., Vodop'yanov S.O., Mishan'kin B.N., Olejnikov I.P., Duvanova O.V. Korrelyaciya mezhdu nalichiem oblasti variabel'nogo tandemnogo povtora VcB i ostrovkom patogennosti VPI-1 u Vibrio cholerae [Correlation between presence of field of variable tandem repetition of VcB and an island of pathogenicity of VPI-1 at Vibrio cholera]. Molekulyarnaya genetika, mikrobiologiya i virusologiya. 2017, vol. 35, no. 2, pp. 49–52. (In Russ.)
2. Kruglikov V.D, Lomov Yu.M., Rozhkov K.K., Mazruho A.B., Mihas' N.K., Monahova E.K., Kaminskij D.I., Ovsova L.M. Vliyanie razlichnyh aminokislot i solej ammoniya v sostave sinteticheskoj pitatel'noj sredy na produkciyu holernogo ehnterotoksina [Influence of various amino acids and salts of ammonium as a part of synthetic nutrient medium on production of a cholera enterotoksin]. Zhurnal mikrobiologii, ehpidemiologii i immunobiologii, 2003, no. 3, pp. 16–21. (In Russ.)
3. Mishan'kin B.H., Vodop'yanov A.S., Lomov Yu.M., Romanova L.V., Vodop'yanov S.O., Suchkov I.Yu., Mishan'kin M.B., Cherepahina I.Ya., Duvanova O.V., Shishiyanu M.V. Retrospektivnyj VNTR-analiz genotipov shtammov Vibrio cholerae O1, vydelennyh na territorii Rostovskoj oblasti v gody VII pandemii holery [The retrospective VNTR analysis of genotypes of strains of Vibrio cholerae O1 allocated for territories of the Rostov region in days of the VII pandemic of cholera]. Molekulyarnaya genetika, mikrobiologiya i virusologiya, 2004, no. 4, pp. 28–33. (In Russ.)
4. Pisanov R.V., Vodop'yanov A.S., Simakova D.I. Rol' malykh RNK v kontrole ehkspressii genov, vovlechennyh v realizeciyu patogennosti Vibrio cholerae [Role of small RNA in control of an expression of the genes involved in realization of pathogenicity of Vibrio cholerae]. Problemy osobo opasnykh infektsiy, 2017, no. 2, pp. 36–39. (In Russ.)
5. Titova S.V., Kruglikov V.D., Ezhova M.I., Vodop'yanov A.S., Arhangel'skaya I.V., Vodop'yanov S.O., Moskvitina Eh.A. Analiz dinamiki vydeleniya i biologicheskikh svojstv shtammov V. cholera O1 El-Tor, izolirovannykh iz vodnykh obe'ktov na territorii Rostovskoj oblasti s 2003–2014 gg. [The analysis of dynamics of allocation and biological properties of the strains V. cholerae O1 El-Tor isolated from water objects in the territory of the Rostov region since 2003–2014]. Zdorovye naseleniya i sreda obitaniya, 2015, no. 2 (263). pp. 39–41. (In Russ.)