Therapeutic Effects of Citric and Succinic Acids in Rats Exposed to Inactivated M. tuberculosis

Abstract

Introduction. The search for methods of correcting pathogenetic disorders related to Mycobacterium tuberculosis, the causative agent of tuberculosis, a highly hazardous communicable and socially significant disease, determines the relevance of the research and its objective to study the role of citric and succinic acids in protective and adaptive processes in warm-blooded animals with connective tissue disorders induced by inactivated mycobacteria. Materials and methods. The study was conducted on male Wistar rats with diseases induced by complete Freund’s adjuvant (a mineral oil emulsion containing heat-killed Mycobacterium tuberculosis). The animals were given a feed-added mixture of organic acids at 17 mg/kg body weight (minimum) and 88 mg/kg body weight (maximum) for 4 weeks. Hematology and bio�chemistry tests were performed using standard methods. The activity of succinate dehydrogenase in blood lymphocytes was determined by the cytobiochemical method. X-rays were obtained using stationary veterinary imaging equipment. Results. The protective effect of carboxylic acids in the exposed animals with Freund’s adjuvant-induced leukocytosis (expressed by a 28 % increase in white blood cells compared to the negative control, p < 0.05), oxidative stress (expressed by an increase in the concentration of malondialdehyde (MDA) by 40 %, p < 0.001, and in inhibition of catalase by 4 %), and subchondral bone sclerosis was characterized by a dose-dependent reduction in immunotoxic manifestations of the disease such as normalization of the number of white blood cells (p < 0.05 compared to model animals); a 27 % reduction in MDA, p < 0.001, a 10 % catalase activation, p < 0.01; succinate dehydrogenase normalization, and a decrease in dystrophic changes in the articular system of animals. Conclusion. The results of hematological, biochemical and radiological tests prove that pathological biochemical and morphological changes related to administration of inactivated M. tuberculosis to warm-blooded animals can be modified by a mixture of citric and succinic acids added to feed, which allows a better understanding of the pathogenesis and an increased therapy effectiveness.