Author:
Amjad Asma,Ansari Farheen,Iqbal Ahsan,Ain Qurat ul,Ali Ahsan,Aziz Sadia,Yusuf Zeeshan
Abstract
GeneXpert is attributed as one of the latest technical means for diagnosing Tuberculosis in very short period of time. Objectives: To evaluate the efficiency of GeneXpert and fluorescent microscopy in the detection of pulmonary tuberculosis (TB); To compare the sensitivity and specificity of GeneXpert and fluorescent microscope in the diagnosis of Mycobacterium tuberculosis Methods: In the present investigation, the diagnostic performance of GeneXpert MTB / RIF for tuberculosis was compared with the performance of light-emitting diode fluorescence microscope in TB samples from Narowal, Pakistan. For this purpose, a total of 299 TB positive specimens were obtained. Among these 54% (n = 160) were categorized to be obtained from male and 46% (n = 139) from female population. Data collected was distributed in 4 age groups; 0-20, 21-40, 41-60, and 61-80, in which the percentage and number of samples were found as 2% (n = 6), 60% (n = 179), 27% (n = 80) and 11% (n = 34), respectively. Results: The parameters including sensitivity and specificity calculated for GeneXpert were 73% and 100%, correspondingly, while the sensitivity and specificity calculated for LED-FM microscope were 43% and 100%, respectively. Conclusions: This indicates that the GeneXpert is more sensitive in detecting MTB in comparison to LED-FM technique. The GeneXpert assay was also found to detect small number of bacillus from samples in comparison to LED-FM method
Publisher
CrossLinks International Publishers
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