Effects of Chlorpyrifos on biochemical characteristics of Labeorohitafish during acute and chronic exposure

Abstract

The aim of present study was to examine the biochemical characteristics of Labeorohita fish during acute and chronic exposure of pesticide, chlorpyrifos (CPF). Methods:During acute exposure, fish were exposed to different concentrations of CPF ranging from 0, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04 and 0.05 mg/L for 96 hrs in glass aquaria. The 96 hrs LC50 value of CPF for Labeorohita was found to be 0.01 mg/L. During chronic exposure fish were subjected to 1/3rd, 1/5th, 1/7th and 1/9th of LC50 for 30 days. At the end of the trial, samples were collected for biochemical (Blood cells, haemoglobin, plasma glucose and plasma protein) and sent to related laboratories. Results:The present study showed the RBCs, haemoglobin and Plasma Glucose level decreases as the concentration of CPF increases. On the other hand, platelets, WBCs and Plasma glucose level increases as the concentration of CPF increases. Decrease in RBCs count and haemoglobin indicates that CPF can cause anaemia to the fish. Conclusions: It is therefore concluded that Chlorpyrifos adversely affects the major organs of the fish Labeorohita.