Susceptibility Pattern of Pathogenic Bacteria “Pseudomonas aeruginosa” to various Antibiotics isolated from Post-Surgical Wound of Diabetic Patient at Hayatabad Medical Complex (HMC) Peshawar

Abstract

Pseudomonas aeruginosa is a gram-negative rod shape opportunistic bacteria involved in nosocomial infection and resistant to most of the commonly used antibiotics. Objective: To find out the antibiotic susceptibly pattern of P. aeruginosa. Methods: The current study was carried out on a sample of Diabetics patients having post-operative wounds in Hyatabad medical Complex. The pus sample was screened of pathogen bacteria P. aeruginosa was isolated from most of the sample i.e. 56% while other microorganisms isolated were i.e.  E coli, S. aureus, Proteus volugarus. Klebseilla spp coliform, Aclnelobacter spp, Proteus merabillus Provedentia spp. For the identification, different tests were performed including culture growth characteristic Gram staining, biochemical test. Antibiotics susceptibility test where performed utilizing disc diffusion method on Mueller Hinton ager. Results: Out of 200 samples, 112 (56%) sample were positive for P. aeruginosa. Infection rate was high in male than female (2:1). The date of the admitted patients in hospital show higher chance of infection as compare of out-patient department (OPD). Among the bacterial isolated 67% where resistant and 31 % were sensitive to cefotaxime, 38% resistance while 61% were sensitive to ceftazidime, 15% resistance and 80 % were sensitive to Meropenem. 10 % resistance to and 86% sensitive to lmipenem, 3.5% resistance and 96% sensitive to tazocin and 6.3 % resistant while 87% were sensitive to sulzon, using disc diffusion method. Conclusions: Meropenem, lmipenem, tazocin and sulzon were formed to be the most effective agents isolated P. aeruginosa isolates. The results of the bacterial isolates did not showed 100% susceptibility against any of the tested antibiotics. In this study, it is concluded that Tazocin and Sulzon is the choice of drug for the SSI of diabetic patients.