Antibacterial Susceptibility Pattern of Gram Negative Bacteria Isolated from Patients with Urinary Tract Infection

Abstract

In clinical setting, urinary tract infections (UTIs) rank second among infectious presentations. Each year, around 150 million people receive a UTI diagnosis worldwide. Objectives: To determine the antibacterial sensitivity of meropenem, imipenem and ciprofloxacin against bacteria isolated from patients presented with Urinary Tract Infection. Methods: This Descriptive type Cross-sectional study was carried out at Clinical Microbiology Laboratory, Pathology Department of King Edward Medical University. A total of 100 patients were included that had Urinary Tract Infections. Urine culture was done using standardized Cysteine lactose electrolyte deficient (CLED) agar. Gram reaction, morphology, and biochemical properties were used to identify bacterial pathogens. Antibiotic sensitivity of the selected Gram negative bacteria was observed by using the Kirby Bauer method for antibiotic susceptibility. Results: The bacteria isolated were E. coli (62%), Klebsiella spp. (11%), Candida spp. (8%), Pseudomonas aeruginosa (7%), Proteus spp. (4%), Staphylococcus spp. (3%), Staphylococcus saprophyticus (3%) and Enterobacter spp. (2%). Sensitivity pattern for Ciprofloxacin was 20 (23.3%) sensitive and 66 (76.7%) resistant. Similarly 87.2% were sensitive to Meropenem and 12.8% were resistant. While for Imipenem 95.3% were sensitive and 4.7% were resistant. Conclusions: It was concluded that UTI most commonly caused by E. coli which is a gram negative bacteria followed by Klebsiella, Pseudomonas, Proteus and Enterobacter. Other microorganisms such as Staphylococcus spp., Staphylococcus saprophyticus and Candida spp. were also isolated in least amount. Antimicrobial susceptibility showed that Gram negative isolates of uropathogens were highly resistant against Ciprofloxacin and showed sensitivity against Imipenem and Meropenem