Effect of Different Media Types on In Vitro Wheat Germination

Abstract

Wheat is a very crucial cereal for us as we highly depend on it and in Pakistan it is a crop which is mostly consumed. Wheat prevents colon cancer as it is a good source of dietary fiber. In vitro plant tissue culture is an alternative way to produce high yielding crops. Therefore, through the in vitro propagation of the wheat we can produce wheat with better quality and high nutritional value. Objective: To determine the rate of germination of the seeds of a wheat cultivar on different types of nutrient media.  Methods: In the current study, two different types of media containing different hormones were used to investigate their effect on seed germination. For this purpose, M1 media with 2,4-D and M2 media with a combination of 2,4-D and BAP was used. Plant growth regulators were added in order to induce the germination process. The wheat cultivar Galaxy-2013 was used where 60 seeds were used for each medium. Results: The germination frequency on M1 was 41.66% while the germination frequency on M2 media was 61.66%.  Conclusions: Hence, the results indicate that the combination of the PGRs showed better germination on MS media. This experiment could help in the selection of media which have a better germination capability for future research.