Determination of Physiological, Biochemical and Anti-oxidative Status in Type 1 Diabetes Mellitus Patients

Abstract

Type 1 Diabetes Mellitus (T1DM) disorganization of glucose equilibrium distinguishes by autoimmune disruption of the insulin producing pancreatic β-cell that constantly leads to insulin scarcity and resulting hyperglycemia Objective: To determine the physiological, biochemical, and anti-oxidant status in Type 1 Diabetes Mellitus Patients. Methods: It is a comparative study. 60 diabetic patients and 50 Samples of healthy individuals were taken from Nawaz Sharif Hospital. Blood samples (5.0 ml) were obtained and centrifuged at 4000 rpm for 10 minutes to separate the serum. Glutathione (GSH), Catalase (CAT), Superoxide Dismutase (SOD), Malondialdehyde (MDA), Nitric oxide (NO), micronutrients (Vitamin A, Vitamin C and Vitamin E) and Electrolytes was determined. Results: MDA level is progressively higher in T1DM (14.01±0.06) as compared to control group (1.27±0.21) (P- Value 0.000). GSH status is notably reduced in diabetic patients (0.15±.05) as compared to normal (6.24±0.33). Comparable anti-oxidant catalase is reduced (2.82±.04) in affected individuals as compared to normal individuals 4.19±1.09. SOD level was remarkably marked up to (13.52±3.21) in susceptible persons as compared to normal (2.15±0.23).  Vitamin A level was markedly reduced to (1.62±0.26) in patients as compared to healthy individuals (7.18±0.33). Conclusions: T1DM patients particularly showed reduced amounts and competency of antioxidant protections due to elevated consumption of specific anti-oxidant components such as low level of intracellular glutathione and Catalase and primarily low levels of vitamin A, vitamin E and vitamin C and exalted level of MDA, SOD and NO.