Specific Parameters of Detection of Fungi of the Genus <i>Aspergillus</i> in Respiratory Organs of Pulmonary Tuberculosis Patients-Reference-Cited by-同舟云学术

Specific Parameters of Detection of Fungi of the Genus <i>Aspergillus</i> in Respiratory Organs of Pulmonary Tuberculosis Patients

Published:2023-01-12 Issue:12 Volume:100 Page:33-38
ISSN:2542-1506
Container-title:Tuberculosis and Lung Diseases
language:
Short-container-title:Tuberk. bolezni lëgk.

Author:

Panova A. E.¹^ORCID,Lovacheva O. V.²^ORCID,Gracheva A. N.¹,Burmistrova I. A.¹,Naumova T. A.¹,Tyulkova T. E.¹

Affiliation:

1. National Medical Research Center of Phthisiopulmonology and Infectious Diseases

2. National Medical Research Center of Phthisiopulmonology and Infectious Diseases; Russian Medical Academy of On-going Professional Education

Abstract

The objective: to study specific parameters of detection of fungi of the genus Aspergillus in respiratory organs of pulmonary tuberculosis patients.Subjects and Methods. In 2019-2021, the samples of sputum or bronchoalveolar lavage of 520 patients above 18 years old with confirmed pulmonary tuberculosis and HIV-negative status were tested for molds. Also, all patients had their sputum or BAL tested for acid fast mycobacteria (AFB), cultures of M. tuberculosis (MBT), and DNA of tuberculous mycobacteria.Results. Positive culture results for fungi were obtained in 50/520 (9.62%; 95% CI 7.37-12.45, Wilson test) pulmonary tuberculosis patients. Species identification revealed fungi of the genus Aspergillus in 46/50 (92%) of them.In 37/46 (80.43%; 95% CI 66.83-89.35) patients, a single type (one species of Aspergillus) was identified, in 9/46 (19.57%; 95% CI 10.65-33.17) two types were identified. A. fumigatus (16/37 isolates, 43.24%) and A. niger (13/37 isolates, 35.14%) prevailed among single types of cultures. A. niger + A. flavus (3/9 isolates, 33.33%) was the most frequent combination. The ratio of the effectiveness of methods for detecting fungi such as culture/direct microscopy/galactomannan antigen in serum and BAL was 100/84.78/4.35% and 67.39%, respectively. Thus, BAL is more preferable than serum for detection of galactomannan antigen in patients with tuberculosis.Only in 8/46 (17.4%) patients, bacterial excretion was detected by culture, which is typical for the active phase of the course of tuberculosis. In the remaining 38 (82.6% 95% CI 69.28-90.91) patients, no tuberculous mycobacteria were detected by culture, which was typical of stabilization and cure of tuberculosis.

Publisher

LLC "Medical Knowledge and Technologies"

Subject

General Medicine

Reference13 articles.

1. Araviyskiy R.A., Klimko N.N., Vasilyeva N.V. Diagnostika mikozov. [Diagnostics of mycosis]. St. Petersburg, Spb MAPO Publ., 2004, 186 p.

2. Ignatieva S.M., Spiridonova V.A., Bogomolova T.S., Shadrivova O.V., Desyatik E.A., Borzova Yu.V., Khostelidi S.N., Volkova A.G., Popova M.O., Zyuzgin I.S., Kolbin A.S., Klimovich A.V., Zubarovskaya L.S., Afanasiev B.V., Vasilyeva N.V., Klimko N.N. Specific testing of galactomannan in serum and bronchoalveolar lavage of oncohematological patients with invasive aspergillosis. Own data and literature review. Problemy Meditsinskoy Mikologii, 2013, vol. 15, no. 4, pp. 45-51. (In Russ.)

3. Kozlov V.A., Tikhonova E.P., Savchenko A.A., Kudryavtsev I.V., Andronova N.V., Anisimova E.N., Golovkin A.S., Demina D.V., Zdzitovetsky D.E., Kalinina Yu.S., Kasparov E.V., Kozlov I.G., Korsunsky I.A., Kudlai D.A., Kuzmina T.Yu., Minoranskaya N.S., Prodeus A.P. ., Starikova E.A., Cherdantsev D.V., Chesnokov A.B., Shesternya P.A., Borisov A.G. Klinicheskaya immunologiya. Prakticheskoye posobiye dlya infektsionistov. [Clinical immunology. A practical guide for infectious disease specialists]. Krasnoyarsk, Polikor Publ., 2021, 563 p.

4. Nozokomialnaya pnevmoniya u vzroslykh. Rossiyskie natsionalnye rekomendatsii [Nosocomial pneumonia in adults: Russian national guidelines]. B.R. Gelfand, eds., 2nd Edition, reviewed and supplemented, Moscow, Med. Inform. Agentstvo Publ. (AFB), 2016 176 p.

5. Runke M. Fungal infections in immunocompromised patients (epidemiology, diagnosis, therapy, and prevention). Problemy Meditsinskoy Mikologii, 2000, vol. 2, no. 1, pp. 4-16. (In Russ.)