Study of acute toxicity of GIZh-298

Abstract

Assessment of acute toxicity is a necessary stage of preclinical research of the substance GIZh-298. The aim of present research is study of acute toxicity GIZh-298. Methods. GIZh-298 was administered once intraperitoneally to mice at doses 200-330 mg/kg. Equivalent volume of 1 % starch solution was administered to animals of the control groups. Periods of intoxication and death of animals with a detailed description of the observed clinical picture were registered. Euthanasia and pathoanatomical dissection were performed 14 days after drug administration. Results. The median lethal doses were identified: LD50 = 299,6 (279,7 – 320,8) mg/kg in female mice, LD50 = 302,3 (281,5 – 324,6) mg/kg in male mice at intraperitoneal introduction. The morphological view of the internal organs, detected during pathoanatomical dissection of all surviving experimental animals, did not differ from that observed in control animals. Conclusion. It was determined that GIZh-298 at intraperitoneal introduction concerns to low-toxic substances. According to classification Sidorov K.K. GIZh-298 may be related to 4th toxicity class.