Development and validation of a method for porphyrins quantification using HPLC-UV in urine-Reference-Cited by-同舟云学术

Development and validation of a method for porphyrins quantification using HPLC-UV in urine

Published:2022-07-06 Issue:2 Volume: Page:36-45
ISSN:2686-8830
Container-title:Pharmacokinetics and Pharmacodynamics
language:
Short-container-title:Fk&Fd

Author:

Kildyushkin D. A.¹^ORCID,Petukhov A. E.¹^ORCID,Litvin E. A.²^ORCID

Affiliation:

1. Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation (Sechenovskiy University)

2. Federal State Budgetary Institution «Federal research center of pediatric hematology, oncology and immunology named after Dmitry Rogachev» of the Ministry of Heath of the Russian Federation

Abstract

Porphyrias are the group of orphan diseases, related to pathological disruptions of heme biosynthesis. These diseases are hard to diagnose timely and existing methods lack universality. High performance liquid chromatography coupled with ultraviolet detector could be adapted for this purpose via determination of porphyrins in human urine. Waters Acquity UPLC H-CLASS with diode matrix was used in this study. Developed method allows determining the inner standard and 9 biomarkers, each connected to specific stage of heme biosynthesis. Developed method is validated for porphyrias diagnosis.

Publisher

Publishing House OKI

Subject

Pharmacology (medical),Complementary and alternative medicine,Pharmaceutical Science

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