Application of the AlphaScreen and AlphaLisa method in drug development and pharmacokinetic studies-Reference-Cited by-同舟云学术

Application of the AlphaScreen and AlphaLisa method in drug development and pharmacokinetic studies

Published:2022-05-01 Issue:1 Volume: Page:44-54
ISSN:2686-8830
Container-title:Pharmacokinetics and Pharmacodynamics
language:
Short-container-title:Fk&Fd

Author:

Mukhametshina R. T.¹^ORCID,Kopein S. D.¹^ORCID,Simonov V. M.¹^ORCID

Affiliation:

1. Joint Stock Company "GENERIUM"

Abstract

Drug development requires high-tech, simple, and sensitive methods. AlphaLISA method was announced as a universal method that would fit the listed criteria. However, research of other works on the pharmacokinetics of drugs related to this method showed a small number of pharmacokinetic studies in clinical trials. In this review, we focused on not only the positive aspects of the Alpha method, but also its disadvantages.

Publisher

Publishing House OKI

Subject

Pharmacology (medical),Complementary and alternative medicine,Pharmaceutical Science

Reference43 articles.

1. Waller H., Chatterji U., Gallay P. et al. The use of AlphaLISA technology to detect interaction between hepatitis C virus encoded NS5A and cyclophilin A. J Virol Methods. 2010; 165 (2): 202–210. DOI: 10.1016/j.jviromet.2010.01.020.

2. Vickers T. A., Crooke S. T. Development of a Quantitative BRET affinity assay for nucleic acid-protein interactions. PLoS One. 2016; 11 (8): e0161930. DOI: 10.1371/journal.pone.0161930.

3. Guenat S., Rouleau N., Bielmann C. et al. Homogeneous and nonradioactive high-throughput screening platform for the characterization of kinase inhibitors in cell lysates. J Biomol Screen. 2006; 11 (8): 1015–1026. DOI: 10.1177/1087057106294697.

4. Gil'miyarova F. N. Belok-ligandnye vzaimodeistviya: vliyanie minornykh komponentov metabolizma / F. N. Gil'miyarova [i dr.] // Siberian Medicl Review. – 2017. – (6): 12–21. [Gylmiyarova F. N., Ryskina E. A., Kolotieva N. A., Potekhina V. I., Gorbacheva I. V. Protein-ligand interactions: the influence of minor components of metabolism. Siberian Medicl Review. 2017; (6): 12–21. (In Russ).]. DOI: 10.20333/2500136-2017-6-12-21.

5. Coussens N. P., Auld D., Roby P. et al. Compound-mediated assay interferences in homogenous proximity assays. Assay Guidance Manual. Review. 2020; NBK553584.