Study of Molecular Parameters and Antibacterial Activity of a Peptide Preparation Derived from Human Leukocytes-Reference-Cited by-同舟云学术

Study of Molecular Parameters and Antibacterial Activity of a Peptide Preparation Derived from Human Leukocytes

Published:2024-01-15 Issue:9-10 Volume:68 Page:20-24
ISSN:0235-2990
Container-title:Antibiotics and Chemotherapy
language:
Short-container-title:A&Ch

Author:

Volkov A. G.¹,Kononova L. I.²,Korobov V. P.³,Volkova L. V.⁴

Affiliation:

1. Perm State Medical University named after Academician E. A. Wagner

2. Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences

3. Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences; Perm National Research Polytechnic University

4. Perm National Research Polytechnic University

Abstract

Background. The study of new antimicrobial substances isolated from the living organism tissues is of particular importance for medicine and the biopharmaceutical industry. Leukocyte peptides can act as an alternative to traditional antibiotics to overcome the bacterial antibiotic resistance problem.Aim. Evaluation of molecular weight and biological activity of ultrasound-derived leukocyte peptides.Methods. Separation of leukocyte peptide complexes into particular fractions by reverse phase chromatography and evaluation of their antibacterial activity by serial dilution and diffusion methods.Results. Studies have shown that one peptide fraction with a molecular weight of 440–570 Da has pronounced antibacterial activity.Conclusion. The obtained experimental data testify to the high therapeutic potential of the leukocyte protein-peptide complex, which has a wide spectrum of antibacterial activity.

Publisher

Publishing House OKI

Reference26 articles.

1. Budihina A.S., Pinegin B.V. Defenziny — mul'tifunkcional'nye kationnye peptidy cheloveka. Immunopatologiya, Allergologiya, Infektologiya. 2008; 2: 31. (in Russian)]

2. Baltzer S.A., Brown M.H. Antimicrobial peptides — promising alternatives to conventional antibiotics. J Mol Microbiol Biotechnol. 2011; 20 (4): 228–235. doi: 10.1159/000331009.

3. Navashin S.M., Sazykin Yu.O. Antibiotiki: novye mekhanizmy peredachi rezistentnosti. Antibiotiki i Khimioter = Antibiotics and Chemotherapy. 1998; 6: 3–6. (in Russian)]

4. Tapal'skij D.V., Mozgova A.V., Kozlova A.I. Effektivnost' kombinacij antibiotikov v otnoshenii karbapenemrezistentnyh gospital'nyh izolyatov Acinetobacter baumannii. Klinicheskaya Infektologiya i Parazitologiya. Specvypusk v Belarusi 2014; 95–103. (in Russian)]

5. Shaginyan I.A., Dmitrienko O.A. Molekulyarnaya epidemiologiya infekcij, vyzyvaemyh meticillinustojchivymi stafilokokkami. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii. 2003; 3: 99–109. (in Russian)]