Affiliation:
1. K. L. Khetagurov North Ossetian State University; North Caucasus Federal University
2. K. L. Khetagurov North Ossetian State University;
3. K. L. Khetagurov North Ossetian State University
4. National Research Center «Kurchatov Institute»
5. Kuban State Technological University
Abstract
The sensitivity of field cultures of fungal microbe Aspergillus niger to new synthetic analogs of natural antifungal antibiotics of the isocoumarin class was determined in comparison with antimycotic activity of known drugs. The minimal mycocidal concentration (MMCC) and minimal mycostatic concentration (MMSC) of the drugs were documented. The inhibitory effect (IE) was expressed as the ratio of the average colony diameter of experimental samples to the average colony diameter of control samples. From the results presented in the present work, it follows that the investigated field cultures of Aspergillus niger are most sensitive to antimycotic drugs belonging to N-methylnaphthylmethylamine derivatives and polyene antimycotics — terbinafine (MMSC = 0–2 µg/cm3) and nystatin (MMSC = 4–8 µg/cm3, IE-1 = 1.69, IE-K2 = 1.51), which have a pronounced mycocidal effect. Other studied preparations, including synthesized analogues of natural isocoumarins, showed only mycostatic effect on cultures of fungal microbe Aspergillus niger: fluconazole — MMSC = more than 64 µg/cm3, IE-K1 = 3.38, IE-K2 = 2.52; griseofulvin — MMSC = more than 64 µg/cm3, IE-K1 = 1.79, IE-K2 = 1.53; 3-(3-oxobutyl)-isocoumarin — MMSC = more than 64 µg/cm3, IE-K1 = 1.27, IE-K2 = 1.07; 7-fluoro-3-(oxobutyl)-1-N-isochromene-1-one — MMSC = greater than 64 µg/cm3, IE-K1 = 1.28, IE-K2 = 1.03; (E)-3-(3-oxobutyl-1-en-1-yl)-1-N-isochromene-1-one — MMSC = greater than 64 µg/cm3, IE-K1 = 1.12, IE-K2 = 1.06; 3-(3,3-difluorobutyl)-1-N-iso-chromene-1-one — MMSC = greater than 64 µg/cm3, IE-K1 = 1.34, IE-K2 = 1.25.
Reference11 articles.
1. Potoroko I.Jyu., Kalinina I.V., Rus'kina A.A. Nauchnye podkhody v obespechenii kachestva i bezopasnosti plodov i ovoshchej v protsesse khraneniya. Mirovoj opyt. Vestnik JyuzhnoUral'skogo gosudarstvennogo universiteta. Seriya: Pishchevye i Biotekhnologii. 2017; 1: 14–18. doi: https://doi.org/10.14529/food170102. (in Russian)
2. Khmeleva E.V., Bakaeva A.N. Vliyanie sposoba podgotovki zerna pshenitsy na pokazateli kachestva i bezopasnosti v tekhnologii zernovogo khleba. Nauchnyj Zhurnal NIU ITMO. Seriya «Protsessy i apparaty pishchevykh proizvodstv». 2016; 4: 3–9. doi: https://doi.org/10.17586/2310-1164-2016-9-4-3-9. (in Russian)
3. Pashchenko L.P., Kolomnikova Ya.P., Ausheva T.A., Pashchenko V.L. Biotekhnologicheskie aspekty v obespechenii mikrobiologicheskoj chistoty pshenichnogo khleba. Vestnik VGUIT. 2012; 1: 87–89. doi: https://doi.org/10.20914/23101202-2012-1-87-89. (in Russian)
4. Sergeev Jyu.V., Shpigel' B.I., Sergeev A.Jyu. Farmakoterapiya mikozov. M.: Meditsina dlya Vsekh. 2003; 200. (in Russian)
5. Skripkin Jyu.K., Kulagin V.I., Leshchenko V.M., Ivanov O.L., Sergeev Jyu.V., Sergeev A.Jyu., Mokina E.V., Tsarev V.N., Trefilov A.G., Tereshchenko A.V. Sravnitel'noe izuchenie protivogribkovoj aktivnosti in vitro original'nogo itrakonazola (orungal) i ego vosproizvedennykh preparatov. Immunopatologiya, Allergologiya, Infektologiya. 2004; 1: 60–65. https://www.immunopathology.com/ru/article.php?carticle=327 (in Russian)