Determination of pyrrolizidine alkoloids in oregano (Origanum onites) produced in Denizli by LC-MS Q-TOF

Author:

KORKMAZ Seda Dicle1,KÜPLÜLÜ Özlem2

Affiliation:

1. GİRESUN ÜNİVERSİTESİ, ESPİYE MESLEK YÜKSEKOKULU

2. Ankara Üniversitesi Veteriner Fakültesi

Abstract

Pyrrolizidine alkaloids (PAs) in certain plant species create contamination, especially in production areas, and pose a risk to public health with the consumption of foods. Due to their sensitivity to intoxications, it has hepatotoxic, pneumotoxic, genotoxic, mutagenic, teratogenic and carcinogenic effects in all age groups, especially in infants and children. In this study, the presence and level of PAs and N-oxide compounds in oregano grown in Denizli, which has an important share in Turkey's oregano export, were investigated by LC-MS Q-TOF to determine potential public health risks. was intended. In the study, a total of 3 processed and packaged oregano samples taken from different enterprises producing oregano in Denizli and 8 unprocessed (stem) oregano samples from different regions of Denizli (11 samples ) were used as material. Identification and quantification of PAs and PANOs compounds in the samples were done by LC-MS Q-TOF. A total of 280 ±22 µg/kg of europine and europine N-oxide were determined in a processed packaged sample from the analyzed samples. According to the analysis results; PA value in oregano samples was determined below the PA limit value (

Publisher

Veteriner Hekimler Dernegi Dergisi

Reference31 articles.

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2. Boppre, M (2011): The ecological context of pyrrolizidine alkaloids in food, feed and forage: an overview. Food Addit Contam Part A, 28(3), 260-281.

3. Chen L, Mulder PPJ, Louisse J, Peijnenburg A, Wesseling S, Rietjens IMCM (2017): Risk assesment for pyrrolizidine alkaloids detected in (herbal) teas and plant food supplements. Regul Toxicol Pharmacol, 86, 292-302.

4. Chen Z, Huo JR (2010): Hepatic veno-occlusive disease associated with toxicity of pyrrolizidine alkoloids in herbal preparations. Neth J Med, 68(6), 252-260.

5. Crews C, Berthiller F, Krska R (2010): Update on analytical methods for toxic pyrrolizidine alkaloids. Anal Bioanal Chem, 396 (1), 327-338.

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