CHROMOSOMIC STUDIES IN ZEPHYRANTHES CITRINA BAKER (AMARYLLIDACEAE), A POLYPLOID ORNAMENTAL

Author:

Daviña J.R.1,Gianini Aquino A.C.1,Rodríguez Mata O.A.1,Tapia-Campos E.2,Barba-Gonzalez R.2,Honfi A.I.1

Affiliation:

1. Programa de Estudios Florísticos y Genética Vegetal (Instituto de Biología Subtropical, IBS, CONICET-UNaM) nodo Posadas, FCEQyN, Universidad Nacional de Misiones, Rivadavia 2370, C.P.3300, Posadas, Misiones, Argentina

2. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Biotecnología Vegetal (CIATEJ) Av. Normalistas 800, Colinas de la Normal, Guadalajara, Jalisco, C.P. 44270. México

Abstract

Zephyranthes citrina is an ornamental American bulbous plant used as an ornamental garden crop for the aesthetic qualities of its yellow perigonium. The objective of this work was to characterize the species by classical chromosome staining and fluorochrome banding. A sporophytic chromosome number of 2n=8x=48 chromosomes was observed, being the karyotypic formula 20 m + 26 sm + 2 st. Satellites were detected in the short arm of metacentric chromosomes 8, 9, 11 and 12, which colocalized with constitutive heterochromatin CMA+/DAPI-/0 bands. The karyotype comprised chromosome pairs with terminal constitutive heterochromatin bands that included satellites and heteromorphic clusters indicating that it is an allooctoploid. These results will be used as a tool for monitoring genetic improvement, in interspecific crosses and its progenies and in biotechnological procedures by in vitro culture. Key words: constitutive heterochromatin, chromosome banding, bulbous, plant genetic resources, karyotype

Publisher

Sociedad Argentina de Genetica

Subject

Genetics (clinical),Genetics,Applied Microbiology and Biotechnology,Biotechnology

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