Absence of the oxytocin-induced prostaglandin F2α secretory response in uterus from ovariectomized ewes and activation of the response in vitro

Author:

Sheldrick E L,Flick-Smith H C,Bendall D E,Flint A P F

Abstract

Abstract Oxytocin-induced prostaglandin F (PGF) responses were measured in explants of uterus from ovariectomized ewes on the day of tissue collection or after culture for 72 h in the presence or absence of oestradiol-17β (100 nmol/l). Oxytocin receptor binding activity was 210 ±47 fmol [3H]oxytocin bound per mg protein in fresh tissue and 89 ± 24 and 90 ± 17 fmol/mg in tissue cultured with control medium or with oestradiol respectively (means ± s.e.m.). PGF production during the hour following oxytocin administration to freshly collected tissue was 272 ± 77 ng/g/h compared with 193 ± 35 ng/g/h in the absence of oxytocin. These rates were 2789 ± 1085 and 353 ± 135 ng/g/h after culture for 72 h in control medium and 2022 ± 496 and 342 ± 134 ng/g/h after culture with oestradiol. Thus oestradiol had no effect on the culture-induced maturation of the PGF response. Short-term exposure to arachidonic acid (66 μmol/l) did not increase PGF production in fresh tissue but significantly increased basal but not oxytocin-induced PGF production after 72 h in culture (P<0·05). There was an absence of oxytocin-induced inositol phosphate turnover in fresh tissue but after culture concentrations of inositol mono-, bis- and trisphosphates were all significandy increased by oxytocin (P<0·005). Antisera directed against G-protein α sub-units αi3, αo, αq, α11 and the common β subunit, and prostaglandin H-synthase-1 detected proteins that were present before and after culture. Oestradiol had no effect on the presence or apparent concentrations of these proteins. Journal of Endocrinology (1995) 145, 299–305

Publisher

Bioscientifica

Subject

Endocrinology,Endocrinology, Diabetes and Metabolism

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