Author:
Tsonis C. G.,McNeilly A. S.,Baird D. T.
Abstract
ABSTRACT
An extremely sensitive and reliable bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro was developed and used to measure exogenous and endogenous inhibin activity in the ewe. The sheep inhibin bioassay is 30- to 40-fold more sensitive than conventional rat inhibin bioassays. The minimum sensitivity of each bioassay in the measurement of inhibin activity in 1 ml of sheep serum is 220 mu. and 4080 mu. in the sheep and rat bioassays respectively. This sensitive inhibin bioassay has permitted, for the first time, the measurement of endogenous inhibin in the peripheral and ovarian vein blood of the sheep, as well as exogenously administered inhibin.
The half-life of exogenously administered ovine inhibin (in follicular fluid) in the sheep was calculated as two components (18–24 and 50–60 min) from the inhibin profiles of six ewes. Inhibin contained in the ovine follicular fluid, given as a bolus i.v. injection, increased to maximum levels after 5 min and then remained increased for 10–32 min depending upon the dose administered, before exponentially decaying. The time for inhibin to exert its effect ranged from 3 to 6 h after injection and appeared to be dose-related. The bolus injection of inhibin, apart from causing suppression of FSH, evoked a large rebound increase of FSH up to 400% of preinjection levels.
The development of the sheep bioassay will allow the measurement of biologically active inhibin in the peripheral circulation and ovarian vein blood of sheep with the possibility of extending this to man.
J. Endocr. (1986) 110, 341–352
Subject
Endocrinology,Endocrinology, Diabetes and Metabolism
Cited by
114 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. New Insights of Inhibins in Ovarian Physiology of Fish;Reviews in Fisheries Science & Aquaculture;2020-01-07
2. Gonadal Peptides;Endocrinology: Adult and Pediatric;2016
3. Diagnostic applications for inhibin and activins;Molecular and Cellular Endocrinology;2012-08
4. Gonadal Peptides;Endocrinology;2010
5. Inhibin and premature ovarian failure;Human Reproduction Update;2009-09-14