Luteinizing hormone responses to repeated injections of luteinizing hormone releasing hormone in the rat during the oestrous cycle and after ovariectomy with or without oestrogen treatment

Abstract

In order to characterize the nature of the LH response to exogenous LH releasing hormone (LH-RH) in female rats during the oestrous cycle and after ovariectomy with or without oestrogen treatment, serum LH levels were determined after repeated LH-RH injections (300 ng/kg body wt, six times with 30-min intervals). The LH response to the first LH-RH stimulation was greatest on the days of pro-oestrus and oestrus followed by dioestrus 2 and dioestrus 1. Second and subsequent LH-RH challenges enhanced the LH response only on pro-oestrus and dioestrus 2. Larger doses of LH-RH (3 μg/kg body wt) induced a small self-priming effect on dioestrus 1 and oestrus. The LH response to the first LH-RH administration increased with time up to 30 days after ovariectomy and then reached a plateau. A small self-priming effect was present in rats ovariectomized for 30 and 60 days, but absent in rats ovariectomized for 5, 10 and 120 days. Oestrogen treatment increased the self-priming effect in rats ovariectomized for 5 days, with little sensitization of the pituitary gland to the first LH-RH injection on the next day. In rats ovariectomized for 120 days, oestrogen treatment enhanced responsiveness to the first and successive LH-RH stimulations on the next day, and further enhancement to the first response only was induced 3 days after oestrogen treatment.