STIMULATION OF CARP INTERRENAL FUNCTION BY ADRENOCORTICOTROPHIN

Abstract

SUMMARY The effect of ACTH on cortisol concentration in the plasma of the carp has been studied; cortisol was determined using a competitive protein-binding radioassay. Blood was sampled at 12.00 h (the diurnal minimum) by cardiac puncture. The plasma cortisol level of undisturbed carp (blood obtained 1–3 min after netting) was 46 ± 14 (s.e.m.) ng/ml. One hour after injection of ACTH (1·0 i.u./100 g body wt., i.m.) the level of cortisol increased to 415 ± 62 ng/ml and reached a maximum of 656 ± 62 ng/ml after 2 h. The level of cortisol in saline-injected control carp after 2 h was 72 ± 33 ng/ml. A transient rise of cortisol was noted in both groups 30 min after injection (315 ± 51, 315 ± 62 ng/ml). This was attributed to a surge of endogenous ACTH, presumably due to handling. Carp head kidneys were superfused in vitro. They released cortisol spontaneously, but the releasing rate declined exponentially and reached a minimum after 135 min. Addition of ACTH to the medium was followed by a prompt increase in cortisol release. The rate of cortisol release was maximal 30–45 min after addition of ACTH and was identical to the rate at the beginning of the superfusion.