EFFECT OF DIBUTYRYL CYCLIC AMP ON THE RELEASE OF MELANOCYTE-STIMULATING HORMONE FROM RAT NEUROINTERMEDIATE LOBES IN VITRO

Abstract

SUMMARY A method for measuring melanocyte-stimulating hormone (MSH) in rat neurointermediate lobe in vitro and in incubation medium, using polyacrylamide gel electrophoresis, is described. Using this technique, it was shown that dibutyryl cyclic AMP increased the release of MSH in vitro, the degree of stimulation depending on the concentration of the nucleotide. The effect of low concentrations of the nucleotide was potentiated by theophylline.