EVALUATION OF HUMAN FOLLICLE-STIMULATING HORMONE PREPARATIONS

Abstract

SUMMARY A procedure for the purification of human pituitary follicle-stimulating hormone (FSH) is outlined. Various methods of protein determination were applied to the preparation (CP 150), and the results obtained compared with those of amino acid analysis. The composition of CP 150 with respect to amino acid and carbohydrate components is reported, and is suggested as the most suitable basis for expression of biological activity and stability. CP 150 sedimented as a single boundary with S20,w = 2·8s. The composition of various reported FSH preparations including CP 150 are compared, and attention is drawn to some striking similarities.