A METHOD FOR THE SEPARATION AND ESTIMATION OF NEUTRAL STEROIDS IN THE URINE OF NEWBORN INFANTS-Reference-Cited by-同舟云学术

A METHOD FOR THE SEPARATION AND ESTIMATION OF NEUTRAL STEROIDS IN THE URINE OF NEWBORN INFANTS

Published:1963-08 Issue:1 Volume:27 Page:31-51
ISSN:0022-0795
Container-title:Journal of Endocrinology
language:
Short-container-title:

Author:

BIRCHALL KATHLEEN,CATHRO D. M.,FORSYTH CONSTANCE C.,MITCHELL F. L.

Abstract

SUMMARY A method is described for the separation and estimation of neutral steroids in infant urine. The free steroids are obtained by a two-stage enzyme hydrolysis. Following preliminary chromatography, the extracts containing steroids of low and medium polarity are suitable for final resolution. The extract containing steroids of high polarity requires further purification by partition between ether-ethanol and aqueous ammonium sulphate, followed by reversed-phase paper chromatography. Final resolution of steroids in fractions of different polarity is achieved using Bush aqueous methanol systems. Losses incurred in the method are estimated by the use of 4-14C steroids.

Publisher

Bioscientifica

Subject

Endocrinology,Endocrinology, Diabetes and Metabolism

Link

https://joe.bioscientifica.com/downloadpdf/journals/joe/27/1/joe_27_1_003.xml

Cited by 29 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Profiling steroid hormones and urinary steroids;Journal of Chromatography B: Biomedical Sciences and Applications;1986-06

2. Simultaneous estimation of urinary steroids by semiautomated gas chromatography. investigation of neo-natal infants and children with abnormal steroid synthesis;Clinica Chimica Acta;1976-06

3. STEROIDS;Paper and Thin Layer Chromatography;1976

4. METABOLIC CONJUGATION AND HYDROLYSIS OF STEROID HORMONES IN THE FETOPLACENTAL UNIT;Metabolic Conjugation and Metabolic Hydrolysis;1970

5. INDIVIDUAL CORTICOSTEROIDS IN THE URINE OF CHILDREN;Journal of Endocrinology;1969-07