Author:
DESHPANDE N.,BULBROOK R. D.
Abstract
SUMMARY
A simple method for the simultaneous estimation of 17-hydroxycorticosteroids and 17-oxosteroids in human plasma is described. It involves extraction of free corticosteroids, hydrolysis of glucuronosides with β-glucuronidase and solvolysis of sulphates, chromatography on silica gel and colorimetry using microcells. Recoveries of added steroids, using appropriate conjugated compounds were over 80%.
Subject
Endocrinology,Endocrinology, Diabetes and Metabolism
Cited by
21 articles.
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