EFFECT OF OVINE LUTEINIZING HORMONE AND PROLACTIN ON PROGESTERONE SECRETION BY GOAT GRANULOSA CELLS IN CULTURE

Abstract

Effects of LH and prolactin on cultures of granulosa cells from the goat were analysed in terms of the secretion of progesterone. The cultures were maintained for 120 h during which time they were treated at 24 and 72 h after explantation with LH and prolactin. Progesterone secreted in the culture medium was determined by radioimmunoassay. The secretion of progesterone by the cultures in response to LH and prolactin was significantly increased (P<0·01) compared with that by control cultures. The pattern of steroid secretion in the two cases (LH-treated and prolactin-treated cultures) was, however, different. The secretion dropped appreciably when the concentration of hormones was increased to 100 mu./ml.