Author:
Nadhirah Nurul,Maideen Haja,Zuraida Ab Rahman,Nazreena Othman Ayu
Abstract
Diplazium simplicivenium Holtt. a species of fern in Peninsular Malaysia. This study reports an efficient method for D. simplicivenium spore sterilisation and the effect of plant growth regulators (PGRs) via green globular bodies (GGB). Sterilisation with 0.1% mercuric chloride effectively allowed spore germination. The result showed that the culture media supplemented with 0.5 mg/L gibberellin positively affects the weight of GGB and the number of shoots with significant differences (ANOVA, p < 0.05). The soaking technique established in this study for spore sterilisation is an efficient approach, and the optimal plant and concentration of plant hormones were identified. This procedure can be applied to other indigenous ferns and closely similar species.
Publisher
Universiti Putra Malaysia
Reference19 articles.
1. Abrori, F. M., Saraswati., Wijarini, F., & Fatmawati. (2022). Introducing the ferns through comics: Visualisation of ethnopteridology study of Dayak Lundayeh tribe. In IOP Conference Series: Earth and Environmental Science (Vol. 1083, No. 1, p. 012013). IOP Publishing. https://doi.org/10.1088/1755-1315/1083/1/012013
2. Babenko, L. M., Romanenko, K. O., Shcherbatiuk, M. M., Vasheka, O. V., Romanenko, P. O., Negretsky, V. A., & Kosakivska, I. V. (2018). Effects of exogenous phytohormones on spore germination and morphogenesis of Polystichum aculeatum (L.) Roth gametophyte in vitro culture. Cytology and Genetics, 52, 117–126. https://doi.org/10.3103/S0095452718020032
3. Chou, H.-M., Huang, Y.-M., Wong, S.-L., Hsieh, T.-H., Hsu, S.-Y., & Chiou, W.-L. (2007). Observations of gametophytes and juvenile sporophytes of Archangiopteris somaí Hayata (Marattiaceae), an endangered fern in Taiwan. Botanical Studies, 48, 205-213.
4. Dyer, A. F. (1979). The experimental biology of ferns. Transactions of the Botanical Society of Edinburgh, 43(2), 75-90. https://doi.org/10.1080/03746607908685341
5. Fernández, H., & Revilla, M. A. (2003). In vitro culture of ornamental ferns. Plant Cell, Tissue and Organ Culture, 73, l-13. https://doi.org/10.1023/A:1022650701341