Author:
Qiao Er Wong Stephanie,Haradzi Najwa Amalina,Sriskanda Dahmendra,Subramaniam Sreeramanan,Lynn Chew Bee
Abstract
Meyer lemon (Citrus × meyeri) is a hybrid citrus fruit from the Rutaceae family, originating from China. It is well-known for its distinctive appearance and flavor, as well as its health-nourishing nutrients. Micropropagation is an efficient alternative in the multiplication of plant stocks suitable for the commercial scale. The inclusion of organic additives in culture media has been found to provide a cost-effective option as a plant growth stimulant for in vitro plant development. The current study intends to assess the effects of coconut water and banana homogenate in the regeneration of Meyer lemon. In vitro, shoots were treated in half-strength Murashige and Skoog media fortified with 2 mg/L 6-benzylaminopurine with varying concentrations of coconut water and banana homogenate without sucrose. Results revealed that the treatment of 30% coconut water and 40 g/L banana homogenate resulted in the greatest proliferation of new shoots (3.00 ± 0.873 and 1.57 ± 0.297, respectively), whereas treatment of 40% coconut water resulted in the greatest shoot elongation of 0.239 ± 0.026 cm. The current study suggested the incorporation of coconut water and banana homogenate as potential substitutes for carbon sources and growth stimulants in the regeneration of Meyer lemon.
Publisher
Universiti Putra Malaysia
Reference41 articles.
1. Adubofuor, J., Amoah, I., Batsa, V., Agyekum, P. B., & Buah, J. A. (2016). Nutrient composition and sensory evaluation of ripe banana slices and bread prepared from ripe banana and wheat composite flours. American Journal of Food and Nutrition, 4(4), 103-111. https://doi.org/10.12691/ajfn-4-4-3
2. Ahloowalia, B. S., Prakash, J., Savangikar, V. A., & Savangikar, C. (2002). Plant tissue culture. In Proceedings of A Technical Meeting (pp. 3-10). International Atomic Energy Agency. https://www-pub.iaea.org/mtcd/publications/pdf/te_1384_web.pdf
3. Al-Khateeb, A. A. (2008). Regulation of in vitro bud formation of date palm (Phoenix dactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology, 99(14), 6550-6555. https://doi.org/10.1016/j.biortech.2007.11.070
4. Al-Khayri, J. M. (2010). Somatic embryogenesis of date palm (Phoenix dactylifera L.) improved by coconut water. Biotechnology, 9(4), 477-484. https://doi.org/10.3923/biotech.2010.477.484
5. Apensa, V., & Mastuti, R. (2018). Effect of banana homogenate on shoot regeneration of ciplukan (Physalis angulata L.). The Journal of Experimental Life Science, 8(1), 53-60. https://doi.org/10.21776/ub.jels.2018.008.01.09
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献