Determination of starch and amylose contents in various cereals using common model of near-infrared reflectance spectroscopy

Author:

He Mohe,Hu Jiaqi,Wu Yanwen,Ouyang Jie

Abstract

Near-infrared reflectance spectroscopy (NIRS) was used to determine the total starch and amylose contents in various kinds of cereals namely wheat, waxy rice, non-waxy rice, millet, sorghum, waxy maize, buckwheat, barley, and hulless oat. The partial least-squares (PLS) analysis and principal component regression (PCR) were used to establish the calibration models. PLS model achieved a better effect than PCR at 1100 - 2500 nm, and the coefficient of determination (R2) of the calibration and prediction sets were both higher than 0.9 after the best pre-treatment method, first derivative plus Savitzky-Golay. Additionally, the root mean square error (RMSE) was lower than 2.50, and the root mean square error of cross-validation (RMSECV) was less than 3.50 for starch. By comparing PLS models at different waveband regions, the optimal determination results for starch and amylose were obtained at 1923 - 1961 and 1724 - 1818 nm, respectively. NIRS was found to be a successful method to determine of the starch and amylose contents in various cereals.

Publisher

Universiti Putra Malaysia

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