Optimisation of biotransformation conditions of theaflavin-3’-gallate-Reference-Cited by-同舟云学术

Optimisation of biotransformation conditions of theaflavin-3’-gallate

Published:2024-04-30 Issue:2 Volume:31 Page:387-397
ISSN:2231-7546
Container-title:International Food Research Journal
language:en
Short-container-title:IFRJ

Author:

Wu Peng,He Ruying,Chen Hui,Gan Qing,Chen Yan,Zhang Xiaoman,Hu Ting,Li Shiming

Abstract

Theaflavin-3’-gallate was synthesised using polyphenol oxidase (PPO) from gallocatechin (EGC) and epicatechin gallonic acid (ECG). Using PPO, Box-Behnken design, and single factor test, the optimal reaction conditions were determined: ECG/EGC ratio of 3:7, magnetic stirring speed of 200 rpm, reaction temperature of 37°C, and enzyme concentration of 20 mg/100 mL. Under these conditions, the yield of TF-3'-G was 18.1%. These parameters represent the optimal conversion conditions for theaflavin-3’-monogallate.

Publisher

Universiti Putra Malaysia

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