Collagen peptide chelated zinc nanoparticles from tilapia scales for zinc supplementation

Abstract

Collagen peptide chelated zinc (CPCZ) nanoparticles were prepared using collagen peptide hydrolysate from tilapia scales. The nano-chelating ability of collagen peptide hydrolysates treated with ten enzymes was evaluated. Among these collagen peptide hydrolysates, trypsin hydrolysate exhibited the best nano-chelating ability. The molecular weight distribution of trypsin hydrolysate that produced CPCZ nanoparticles indicated that most of the peptides were less than 1,000 Da. These small molecular peptides with excellent nanozinc-chelating ability were chelated with zinc ions to form CPCZ nanoparticles. CPCZ nanoparticles were nearly spherical with an average diameter of approximately 100 nm, and a zinc content of 13.2%. Transmission electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectrometry was used to measure the physicochemical properties of the CPCZ nanoparticles. Their cytotoxicity was also estimated by BHK21 cells. Result indicated that the CPCZ nanoparticles were non-toxic to BHK-21, and such nanoparticles significantly enhanced the survival of cells. The present work suggested that CPCZ nanoparticles could be used as zinc supplementation in the food and pharmaceutical industries.