Wound treatment Q-switched Nd: YAG Laser tattoo removal rat model by Photobiomodulation Therapy PBMT

Abstract

The Q-switched laser method is the most effective method of tattoo removal compared to other methods of i.e. chemical, mechanical and surgical. The objective of this study is to investigate the effect of Nd:YAG laser to remove the tattoo and also to utilize 808 nm diode laser to enhance the wound healing at post-treatment. Q-switched Nd: YAG laser with fundamental wavelength of 1064 nm is applied to remove tattoo pigment with different energy fluencies. Additionally, 808 nm diode laser will be applied on wound created after tattoo removal to enhance the healing process. More than 15 Sprague Dawley rats will be involved and tattooed with black inks on their back by a tattoo gun. A single session treatment is use in the energy about 400mj for tattoo removal. The exposure to diode laser treatment will be conducted daily. The experimental observation will be hold for 9 days, and 5 rats from each group will be sacrificed on day 3, 6 and 9 respectively. The skin biopsy will be removed for H&E and Masson’s trichrome histology examination. The effect of tattoo removal and healing progress of skin analyzed via photothermal.. The preliminary findings of this study indicate that 1064 nm wavelengths of Q-switched Nd-YAG laser treatment with 400mj pulse energy exhibit significant progress of black tattoo removal.